Process for the preparation of 2,5-dideoxystreptamine and of a novel intermediate therefor

ABSTRACT

The aminocyclitol 2,5-dideoxystreptamine may be prepared by a novel two-step synthesis from cis-4,8-dioxatricyclo [5.1.0.0 3 ,5 ]octane. The aminocyclitol and a novel intermediate in the synthesis thereof are utilized by a Micromonospora inyoensis (M. inoyensis strain 1550F-1G NRRL 5742) for the elaboration of a novel antibacterial agent designated Mutamicin 2.

This is a division of application Ser. No. 443,051, filed Feb. 15, 1974now U.S. Pat. No. 4,092,359.

This invention relates to 6,7,-diaza-2,4-dihydroxybicyclo [3.2.1] octaneand 2,5-dideoxystreptamine. More particularly, this invention relates tothe above named novel compounds and to a novel chemical process for thepreparation thereof wherein cis 4,8-dioxatricyclo [5.1.0.0³,5 ] octane(hereinafter cis 1,4-cyclohexadiene dioxide) is utilized as the startingcompound. This invention also relates to acid addition salts of thecompounds and to methods for preparing said salts.

PRIOR ART

Many aminoglycoside antibiotics contain subunits which areaminocyclitols. The aminocyclitol most frequently encountered as asubunit is 2-deoxystreptamine, the compound being one of the subunits ofgentamicin, kanamycin, neomycin, paromomycin, and sisomicin. Recently,W. Thomas Shier and coworkers in the Proceedings of the National Academyof Science 63:198-204 (1969), reported that strains of certainmicroorganisms could utilize certain aminocyclitols to produceantibiotics, some of which are novel.

However, in order to effect such a process, it is necessary to devisemethods for synthesing the aminocyclitols since many of the ones alreadyknown in the art are produced as a subunit of an antibiotic and areavailable only by degradation of an aminocyclitol antibiotic which wouldrender the aminocyclitol prohibitively expensive.

The preparation of cis 1,4-cyclohexadiene dioxide, the starting materialfor the synthesis of the compounds of this invention, is described in apaper by Craig, T. W. et al. in the Journal of Organic Chemistry, Vol.32, pages 3743-3749 (1967).

In an application by M. J. Weinstein et al. entitled, "New AntibioticsProduced by Micromonospora Mutants" Ser. No. 443,052, being filedconcurrently herewith, is described a process by which Micromonosporainyoensis strain 1550F-1G NRRL 5742 utilizes 2,5-dideoxystreptamine toprepare a novel antibiotic designated mutamicin 2.

Mutamicin 2 is a broad spectrum antibiotic having the followingstructure, the 2,5-dideoxystreptamine subunit being the one shown in theupper right side of formula I. ##STR1##

Since Mutamicin 2 is a broad spectrum antibacterial agent, it may beused to inhibit or destroy susceptible bacteria and is especially usefulin admixture with soaps and detergents for cleaning hospital equipmentand laboratory glassware.

As stated above, when 2,5-dideoxystreptamine is added to a fermentationmedium containing Micromonospora inyoensis strain 1550F-1G NRRL 5742 anda assimilable source of carbon and nitrogen, as described in theabove-mentioned patent application, said mutant produces Mutamicin 2. Inthe absence of 2,5-dideoxystreptamine or other aminocyclitol, theaforesaid mutant produces no detectable quantity of antibiotic.Therefore, this compound is necessary intermediate for the production ofMutamicin 2 and may also be used as a building block in otherbiosynthetic or chemical syntheses.

SUMMARY AND DESCRIPTION OF THE INVENTION

The invention sought to be patented may be described as a two-stageprocess for preparing 2,5-dideoxystreptamine (II) ##STR2## (A) Treatingcis-1,4-cyclohexadiene dioxide (III) with hydrazine to form6,7-diaza-2,4-dihydroxybicyclo [3.2.1] octane (IV), (B) hydrogenolyzingsaid compound (IV) to form 2,5-dideoxystreptamine (II) and optionallyconverting the product to an acid addition salt. ##STR3##

The process of Step A is advantageously effected at elevatedtemperatures such as, at the boiling points of such organic solvents astoluene, xylene, 2-ethoxyethanol or preferably, 2-methoxyethanol.Further, the reaction is preferably effected in the presence ofanhydrous magnesium sulfate which acts as a dehydrating agent and mayserve as a complexing agent which promotes the opening of the epoxide inthe desired configuration. Additionally, the reaction is usuallyeffected for from about 18 to about 28, preferably about 24 hours in aninert atmosphere preferably under nitrogen or argon. Thus, the compoundsof this invention embrace 2,5-dideoxystreptamine, and the acid additionsalts thereof. Also embraced herein are 6,7-diaza-2,4-dihydroxybicyclo[3.2.1] octane and acid addition salts of this compound.

The process of steb B is advantageously effected at ambient temperatureand above atmospheric pressure for from about 18 to 28, preferably 24hours. Further, the reaction is most economically effected using wateras the solvent. However, mixtures of water and water miscible organicsolvents which are inert to catalytic hydrogenation, such as alcohols,may also be used. The catalysts employed in the reaction may be themetal catalysts usually employed for hydrogenation or hydrogenolysisreactions such as palladium, platinum, rhodium, or preferably RaneyNickel.

The compounds of this invention are generally used in the form of thefree nitrogen base but may be used in the form of salts of acids such asthe mineral acids, hydrocarbon carboxylic acids or alkyl, aryl oraralkyl sulfonic acid or the like. The formation of such acid additionsalts is usually effected in the conventional manner such as, by theaddition of an excess of acid to a solution of the free base and theprecipitation of the salt by the addition of a precipitating agent.Further, the formation of such salts facilitates the isolation andpurification of the novel compounds of this invention.

The following examples set forth the best mode contemplated byapplicants for carrying out the instant invention:

EXAMPLE 1 2,5-Dideoxystreptamine Dihydrochloride A.6,7-Diaza-2,4-dihydroxybicyclo [3.2.1] octane

Combine and heat at reflux with stirring under an inert atmosphere (N₂or argon) for 24 hours 70 g. of cis-1,4-cyclohexadiene dioxide, 80 ml.of hydrazine hydrate and 80 g. of anhydrous magnesium sulfate in 7000ml. of 2-methoxy-ethanol. Remove the solvent under reduced pressure andextract the residue with hot methanol (1500 ml.). Evaporate the methanolto give the product as a white crystalline solid.

Yield 75 g., m.p. 199°-200° (dec.).

Analysis: Calculated for C₆ H₁₂ N₂ O₂ : C, 49.85; H, 8.25; N, 19.40%.Found: C, 50.15; H, 8.59; N, 19.33%.

B. 2,5-Dideoxystreptamine

Hydrogenate 100 mg. of the product of step A in the 30 ml. of water at42 p.s.i over 20 mg. of a Raney Nickel catalyst for 24 hours. Remove thecatalyst and evaporate the solvent under reduced pressure affording2,5-dideoxystreptamine.

Yield 100 mg., m.p. 187° (dec.).

C. 2,5-Dideoxystreptamine Dihydrochloride

Prepare a solution of 73 mg. of 2,5-dideoxytstreptamine in 5 ml. ofwater, add 1.1 ml. of 1 N hydrochloric acid solution stir for 10minutes. Lyophilize the resulting solution and obtain thereby 106 mg. ofthe title compound which upon crystallization from aqueous ethanolaffords substantially pure 2,5-dideoxystreptamine dihydrochloride, m.p.340° C.

Analysis: Calculated for: C₆ H₁₄ N₂ O₂.2HCL: C, 32.88; H, 7.37; N,12.78%. Found: C, 32.73; H, 7.24; N, 12.31%.

We claim:
 1. A compound of the formula: ##STR4## and the non-toxic acidaddition salts thereof.
 2. An acid addition salt of6,7-diaza-2,4-dihydroxybicyclo [3.2.1] octane.